In addition to its powerful gel-based protein and proteome services NHD offers sophisticated mass spectrometry (MS) services such as

• • Shot Gun MS for protein identifcation in proteomes
• • Targeted MS for ID and validation of biomarkers
• • MALDI-TOF fingerprinting

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NHD mass spec services enables the detection, identification and quantification of proteins by measuring their mass and characterizing their chemical structure.

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About MS

Mass spectrometry in proteomics

In proteomics, mass spectrometry is applied to characterize and sequence proteins. Over the last decades, it has become an indispensable technique. Its development over the last decades has led to three fundamental applications: determining protein interaction, categorizing protein expression and locating protein modification.

Mass spectrometry in pharmacology

In pharmacology, mass spectrometry is applied for drug discovery to determine the structure of drugs and metabolites and for screening metabolites in biological systems.

Tandem Mass Spectrometry (MS/MS)

Tandem mass spectrometry involves a tandem mass spectrometer. The sample undergoes ionization and mass-charge ratio separation in the first stage of mass spectrometry (MS1). Then, selected ions of the articular mass-charge ratio are fragmented to generate product ions for detection in the second stage of mass spectrometry (MS2).

Tandem mass spectrometry gives additional information about the selected ions. It is typically applied to sequence proteins and oligonucleotides because the fragments analyzed can be compared to peptides or nucleic acid sequences found in databases.